into mice (200 μL/mouse), as previously described. Recipient SR‐AI –/– mice were treated with 100 μL of clodronate liposome suspension (Encapsula NanoSciences, Brentwood, TN) by tail vein injection 1 day preceding transfer of 2 × 10 6 differentiated bone‐marrow–derived macrophages (BMDMs), also by tail vein injection. Liposomal clodronate (Clodrosome) and control liposome were pur-chased from Encapsula NanoSciences (Brentwood, TN, USA). Depletion of macrophages in vivo was performed as described previously, with minor modifications. ... Encapsula NanoSciences LLC ... Brentwood, TN 37027 www.encapsula.com. About Us; Contact Us; Database; Routes of Administration. Control mice were injected with 100 μL PBS. Dichloromethylene diphosphonic acid (clodronate)‐loaded liposomes (Encapsula NanoSciences, Brentwood, TN, USA) were injected i.v. In the infarcted heart, clodronate has been applied to specifically study the role of monocytes in the healing process after myocardial infarction [29, 30]. liposomal clodronate i.v. Mirafzali, Z., Thompson, C.S. Contact Encapsula NanoSciences LLC 5409 Maryland Way Suite 360 Brentwood, TN 37027 Phone: 615-884-4442 Fax: 615-250-8747 URL: www.encapsula.com Direct Administration of Clodronate Liposomes into the Cerebrospinal Fluid (CSF) and other Brain Tissue . www.clodrosome.com. 2.2. Liposomal bisphosphonates, BrdU, and anti-CD14 antibody injections. Encapsome® is formulated and prepared identically to Clodrosome® except that clodronate is not added to the liposomes. Clodrosome® is a multilamellar liposome suspension in which clodronate is encapsulated in the aqueous compartments of the liposomes. Recipient SR‐AI –/– mice were treated with 100 μL of clodronate liposome suspension (Encapsula NanoSciences, Brentwood, TN) by tail vein injection 1 day preceding transfer of 2 × 10 6 differentiated bone‐marrow–derived macrophages (BMDMs), also by tail vein injection. Intrarectal Administration of Clodronate Liposomes . The clodronate liposome field, in particular, has been plagued by the introduction of a liposome formulation prepared in the presence of p-aminophenyl mannose, but without covalently coupling this modified sugar to the liposome surface. LC was obtained from Nico van Rooijen at VU University Medical Center (Amsterdam, Netherlands) and formulated as described previously [].LA was manufactured by BioRest (Yavne, Israel) as described previously [17, 18].The appropriate mass of LC or LA (determined by animal body weight) was diluted in room temperature, 1× … Home; The Company. Cyto-kines were purchased … Clodrosome (150 l/mouse; Encapsula NanoSciences, Brentwood, TN, USA) or encapsome (150 µl/mouse, serves as control; Encapsula NanoSciences) 19 was injected i.t. ... Encapsula NanoSciences LLC 5409 Maryland Way. Clodronate (Dichloromethylene Biphosphonic acid), encapsulated in liposomes was purchased from Encapsula nanosciences (Brentwood, TN) along with control empty liposomes. Few details of the the instillation method were provided and it is unclear as to whether a polycarbonate tip on an automatic pipettor or a glass-capillary type micropipette was used for the instillation of the liposome suspension. Encapsula NanoSciences is a specialty company focused on providing liposome research, development and manufacturing expertise in the production of lipid-based nanoparticles, also known as liposomes, to clients and partners in both the academic and industrial sectors. Clodronate‐containing liposomes have been widely used experimentally for the selective depletion of circulating monocytes . Liposomes were composed of 18.8 mg/mL of l-a-Phosphatidylcholine, 4.2 mg/mL of cholesterol, and 0.025 mg/mL of the lipophilic fluorescent probe 3,3′-Dio-ctadecyloxacarbocyanine Perchlorate (DiO, λ ex = 484 nm, λ em = 501 nm, Molecular Probes, USA). Liposome preparation and characterization. For monocytic or granulocytic cells depletion, animals received single vs. continuous (twice per week) intraperitoneal (i.p.) Drug loaded type II immunoliposomes containing antibodies that target an internalized antigen such as CD19 showed much more potency compared to immunoliposomes containing antibodies that target a non internalized antigen such as CD20. Brentwood, TN 37027 www.encapsula.com. Currently, a single paper cited intrarectal administration of clodronate liposomes [1]. Mice were retro-orbitally injected with 200 or 300 μL of a clodronate-loaded liposome suspension (Encapsula NanoSciences, Brentwood, TN) every other day for four doses during the week prior to BM transplantation and beginning immunosuppression. (Clodrosome; Encapsula NanoSciences Brentwood, TN, USA) 24 h before transplantation, and then, 50 µl intrascrotally every 48 h thereafter. Isolation and culture of hepatic macrophages To confirm this, we exploited a method developed for specific macrophage/monocyte depletion using clodronate liposomes. ... Encapsula NanoSciences LLC 5409 Maryland Way. Control mice were given empty liposomes (Encapsome; Encapsula NanoSciences) following the same protocol. Suite 360. injections of clodronate liposomes (2 mg) (Encapsula NanoSciences, Brentwood, TN) or 1A8 rat mAb (1 mg) (BioXCell, West Lebanon, NH), respectively. Liver-resident macrophages (Kupffer cells) were depleted prior to infection through intraperitoneal injection of liposome-encapsulated clodronate (0.2 mL, Clodrosome, Encapsula NanoSciences LLC, Brentwood, TN, USA). (Clodrosome; Encapsula NanoSciences Brentwood, TN, USA) 24 h before transplantation, and then, 50 ml intrascrotally every 48 h thereafter. Suite 360. Rat anti–IFN-γ monoclonal antibody (clone: XMG1.2, BioXcell), clodronate and empty liposomes were from Encapsula NanoSciences (Brentwood, TN). 15. Medium was replaced with fresh differentiation medium every 2 days. The following formulation is used for calculation of number of liposome particles per milliliter of liposome solution. 26,27 Mice were injected retro-orbitally with 200 to 300 μL of a clodronate-loaded liposome suspension (Clodrosome; Encapsula NanoSciences, Nashville, TN) every other day for a total of 4 doses in the week prior to BM failure induction. The brain protects itself from blood-borne pathogens and toxins by severely restricting the transport of cells, particles and most hydrophilic molecules across the vascular endothelium of … Depletion efficiency was determined by microscope examination of CX In this case the validity of the experiment should be seriously questioned. Osmotic pump (Alzet model 1007D, 100 ml; 0.5 ml/h), cannula (Azlet brain infusion kit 3) were from Charles River, Italy. Control mice were given empty liposomes (Encapsome; Encapsula NanoSciences) following the same protocol. A liposome formulator should pay a very close attention to the osmolality of the solution that is encapsulated inside the liposomes, the osmolality of the buffer outside of the liposomes and the osmolality of the medium that is used for in vitro studies. γδ T cells deficient mice (GDKO) were intra-nasally administered with 40 μl of the commercial formulation every 48 … Medium was replaced with fresh differentiation medium every 2 days. Tissues were collected, frozen, and stored at −80 °C until use. Currently, a single paper cited intrarectal administration of clodronate liposomes [1]. Inflammation or necrosis at the injection site of only a few animals suggests contamination during the injection procedure while such reactions in several animals can be an indication that the clodronate liposome suspension has been contaminated. Clodrosome® Liposomal Clodronate Suspension - 5 ml in glass vial Contains: Clodronate ((dichloro-phosphono-methyl)phosphonate) Disodium Salt 5 mg/ml L-α-Phosphatidylcholine 18.8 mg/ml Cholesterol 4.2 mg/ml Suspended in Phosphate Buffered Saline at pH 7.4 2. and Tallua, K, Application of Liposomes in the Food Industry, Encapsula NanoSciences, Brentwood, Tennessee, USA, 2014. where N(a) is the Avogadro number and it is equal to 6.02E23, M (lipid) is the molar concentration of lipid, and N(tot) is the total number of lipids per liposome. Few details of the the instillation method were provided and it is unclear as to whether a polycarbonate tip on an automatic pipettor or a glass-capillary type micropipette was used for the instillation of the liposome suspension. 7-Cl-O-Nec-1 (Nec-1s, 4mg/kg) or zDEVD-fmk (3.3mg/kg) was administered 1 hour prior to the ischemia, i.p. Check the Caspio Server Status. The majority of the clodronate liposome dose which enter the mesenteric and celiac arteries will be ... contamination during the injection procedure while such reactions in several animals can be an indication that the clodronate liposome suspension has been contaminated. Muscle Mϕs were depleted from mice by injection of 200 µl, 5 mg/ml liposomal clodronate i.v. The study with clodronate liposomes (CL) or PBS liposomes (PBSL) (Encapsula NanoSciences, Brentwood, TN, USA) was initiated in 4 week old mice and terminated when the mice reached the age of 8 weeks. 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